School of Medicine

Wayne State University School of Medicine

Recent Publications


Gangcuangco LM, Alejandria M, Henson KD, Saniel M. 
Re: "Antimicrobial Susceptibility of Escherichia coli in Uncomplicated Cystitis in the Emergency Department:  Is the Hospital Antibiogram an Effective Treatement Guide?". 
Acad Emerg Med, 2016, 23(2):215-6


Neelakanta A, Sharma S, Kesani VP, Salim M, Pervaiz A, Aftab N, Mann T, Tashtoush N, Karino S, Dhar S, Kaye KS. 
Impact of Changes in the NHSN Catheter-Associated Urinary Tract Infection (CAUTI) Surveillance Criteria on the Frequency and Epidemiology of CAUTI in Intensive Care Units (ICUs).
Infect Control Hosp Epidemiol. 2015, 36(3):346-9

Henson KE, Levine MT, Wong EA, Levine DP. 
Glycopeptide antiobiotics: evolving resistance, pharmacology and adverse event profile. 
Expert Rev Anti Infect Ther 2015, 13(10):1265-78


Buensaliodo JAL, Chandrasekar PH.
Prophylaxis against hepatitis B reactivation among patients with lymphoma receiving rituximab. 
Expert Rev Anti Infect 2014 Feb;12(2):151-4

Reyes MP, Zhao JJ, Buensalido JAL. 
Current Perspectives: Therapeutic Uses of Tobramycin. 
J Pharmacovigilance 2012, 2:2

Buensalido JAL, DeMarco C, Lerner S. 
Chapter: Mechanism of Action of Antibacterials. 
Practical Handbook of Microbiology 3rd ed
Edited by Emmanuel Goldman  Lorrence H. Green, 2014

Schindler BD, Buensalido JAL, Kaatz GW. 
Chapter: Fluroquinolone Resistance in Bacteria. 
Antimicrobial Drug Resistance 2 ed.
  Edited by Douglas L Mayer, 2014

Buensalido JAL, MacArthur RD. 
Chapter: Clinical Infectious Diseases. 
Sepsis, Severe Sepsis and Septic Shock, 2 ed.
  Edited by David Schlossberg, 2014


Bhargava A, Chopra A, Luigino B, Chopra T. 
Oral Contraceptive causing renal artery thrombosis.
BMJ Case Reports 2013 Jan PMID # 23349178

Marchaim D, Sunkara B, Lephart PR, Gudur UM, Bhargava A, Mynatt RP, Zhao JJ, Bheemreddy S, Hayakawa K, Chopra T, Dhar S, Kaye KS. 
Extended-Spectrum β-Lactamase Producers Reported as Susceptible to Piperacillin-Tazobactam, Cefepime, and Cerfuroxime in the Era of Lowered Breakpoints and No Confirmatory Tests.
Infect Control Hosp Epidemiol 2012 Aug; 33(8):853-5

Hayakawa K, Marchaim D, Bhargava A, Palla MB, Alshabani K, Mahesh U, Pulluru H, Bathina P, Sundaragiri PR, Sakar M, Kakarlapudi H, Ramasamy Balaji, Najreddy P, Mohin S, Dasagi M, Datla S, Kuchipudi V, Reddy S, Shanani S, Upputuri V, Moshos JA, Lephart PR, Toth Martin E, Flanagan E, Pogue J, Kaye K. 
Current Epidemiology and Clinical Impact of Extended-Spectrum Ãc-Lactamase-Producing Escherichia coli at a Tertiary Medical Center. 
American Journal of Infection Control 2012 June; 40(5):e65-e67

Bhargava A, Rao V, Bhargava P. 
Mitral Valve Endocarditis with high level aminoglycoside resistant Enterococcus faecalis in Breast Cancer patient. 
Int Journal of Case Reports and Images, 2012 Sept; 3(9):5-8

Chopra T, Marchaim D, Veltman J, Johnson P, Zhao J, Tansek R, Hatahet D, Chaudhnry K, Pogue J, Rahbar H, Chen TY, Truong T, Rodriguez V, Ellswork J, Bernabela L, Bhargava A, Yousuf A, Alangaden G, Kaye K. 
Impact of cefepime therapy on mortality among patients with blood stream infections caused by extended spectrum β-lactamase-producing Enterobacteriaceae. 
Antimicrob Agents Cheother. 2012 Jul; 56(7):3936-42

Marchaim D, Chopra T, Bhargava A, Bogan C, Tansek R, Abreu-Lanfranco O, Lephart PR, Pogue JM, Hayakawa K, Sunkara B, Dhar S, Perez F, Bonomo RA, Kaye KS. 
Importance of Recent Antimicrobial exposure as a unique predictor for carbapenem-resistant Enterobacteriaceae (CRE) isolation.
Infect Control Hosp Epidemiol. 2012 Aug; 33(8):817-30

Ku K, Moshos J, Bheemreddy S, Wang Y, Bhargava A, Campbell M, Khandker N, Chopra T, Hayakawa K, Abreu-Lanfranco O, Lephart PR, Pogue JM, Dhar S, Kaye KS, Marchaim D. (2011)
Retrospective evaluation of Colistin versus Tigecycline for the treatment of Acinetobacter baumannii and/or carbapenem resistant Enterobacteriaceae infections. 
Am J Infect Contr 2012 Dec; 40(10):983-7

Hayakawa K, Gattu S, Marchaim D , Bhargava A, Palla M, Alshabani K, Mahesh Gudur U. Pulluru H, Bathina P, Rao Sundaragiri P, Sarkar M, Kakarlapudi H, Ramasamy B, Nanjireddy P, Mohin S, Dasagi M, Datla S, Kuchipudi V, Reddy S, Shahani S, Upputuri V, Marrey S, Gannamani V, Madhanagopal N, Annagi S, Sudha B, Muppavarapu KS, Moshos JA, Lephart PR, Pogue JM, Bush K, Kaye KS. 
Epidemiology and risk factors for isolation of CTX-M type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in a large US medical center. 
Antimicrob Agents Chemother 2013 Jun 10

Chinderl B, Jacinto P, Kaatz G.
Inhibition of drug efflux pumps in Staphylococcus aureus: current status of potentiating existing antibiotics.
Future Microbiology  2013 Apr; 8(4):491-507

Jacinto P, Chandrasekar PH. 
Safety of Posaconazole. 
Expert Opin Drug Saf. 2013 Mar; 12(2):265-74

Kosmidis C, Schindler B, Jacinto P, Patel D, Bains K, Seo S, Kaatz G. 
Expression of multidrug resistance efflux pump genes in clinical and environmental isolates of Staphylococcus aureus. 
Int J Antimicrob Agents 2012 Sep; 40(3):204-9

Schindler B, Seo S, Jacinto J, Kumaraswami M, Birukou I, Brennan RG, Kaatz GW. 
Functional consequences of substitution mutations in MepR, a repressor of the staphylococcus aureus mepA multidrug efflux pump gene. 
J Bacteriol. 2013 Jun 7

Suramaethakul N, Chandrasekar PH. 
Band-like Erythema over the Abdomen.
Infectious Diseases in Clinical Practice

Suramaethakul N, Chopra T. 
Clostridium difficile epidemiology: A Global Perspective. 
Future Science Group eBook

Arora NP, Reyes Sacin C, Revankar SG. 
Pulmonary Fungus Ball in a Patient with Sarcoidosis.
The American Journal of the Medical Sciences.  2012 Dec. 28 

Arora NP, Taneja V, Reyes Sacin C, Bhanot R, Natesan SK. 
Coccidioidomycosis masquerading as malignancy. 
BMJ Case Reports.  2012 Feb 25


Ku K, Pogue JM, Moshos J, Bheemreddy S, Wang Y, Bhargava A, Campbell M, Khandker N, Lephart PR, Chopra T, Hayakawa K, Martin ET, Abreu-Lanfranco O, Dhar S, Kaye KS, Marcaim D. 
Retrospective evaluation of Colistin versus tigecycline for the treatment of Acinetobacter baumannii and/or carbapenem-resistant Enterobacteriaceae infections.
Am J Infect Control 2012 Dec; 40(10):983-7, 2012 Mar 21

Collins VL, Marchaim D, Pogue JM, Moshos J, Bheemreddy S, Sunkar B, Shallal A, Chugh N, Eiseler S, Bhargava P, Blunden C, Lephart PR, Memon BI, Hayakawa K, Abreu-Lanfranco O, Chopra T, Munoz-Price LS, Carmeli Y, Kaye KS. 
Efficacy of ertapenem for treatment of bloodstream infections caused by extended-spectrum-β-lactamase-producing Enterobacteriaceae.
Antimicrob Agents Chemother. 2012 Apr; 56(4):2173-7

Marchaim D, Taylor AR, Hayakawa K, Bhemreddy S, Sunkara B, Moshos J, Chopra T, Abreu-Lanfranco O, Martin ET, Pogue JM, Lephart PR, Panda S, Dhar S, Kaye KS. 
Hospital bath basins are frequently contaminated with multidrug-resistant human pathogens.
Am J Infect Control. 2012 Aug;40(6):562-4

Martin ET, Tansek R, Collins V, Hayakawa K, Abreu-Lanfranco O,Chopra T, Lephart PR, Pogue JM,Kaye KS,Marchaim D
The carbapenem-resistant Enterobacteriaceae score: a bedside score to rule out infection with carbapenem-resistant Enterobacteriaceae among hospitalized patients.
Amj Infect Control 2013 Feb;41(2):180-2

Levine DP, Abrue-Lanfranco O. 
MRSA guidelines: a matter of time.
Expert Rev Anti Infect Ther. 2011 May;9(5):495-6

Abreu-Lanfranco O, Levine D. 
Infective Endocarditis in IVD user.
Decision Support in Medicine 1st Edition 2011

Kosmidis C, Schindler BD, Jacinto PL, Patel, Bains K, Seo SM, Kaatz GW. 
Expression of multidrug resistance efflux pump gene in clinical and environmental isolates of Staphylococcus aureus. Int J Antimicrob Agents 2012 Sep;40(3):204-9.

Mitra S, Alangaden GJ. 
Recurrent Urinary Tract Infections in Kidney Transplant Recipients.
Curr Infect Dis Rep. 2011 Dec; 13(6):579-87

Mitra S, Crane L. 
Diagnosis, Treatment and Prevention of Anal Cancer.
Curr Infect Dis Rep 2012 Feb; 14(1):61-6


Flanagan E, Chopra T, Mody L. 
Infection prevention in alternative health care settings.
Infect Dis Clin North Am 2011 Mar; 25(1):271-83.

Saely S, Kaye KS, Fairfax MR, Chopra T, Pogue JM. 
Investigating the impact of the definition of previous antibiotic exposure related to isolation of extended spectrum β-Lactamase-producing Klebsiella pneumoniae.
Am J Infect Control 2011 June; 39(5):390-5.

Marchaim D, Chopra T, Pogue JM, Perez F, Hujer AM, Rudin S, Endimiani A, Navon-Venezia S, Hothi J, Slim J, Blunden C, Shango M, Lephart PR, Salimnia H, Reid D, Moshos J, Hafeez W, Bheemreddy S, Chen TY, Dhar S, Bonomo RA, Kaye KS. 
Outbreak of colistin-resistant, carbapenems-resistant Klesbsiella pneumoniae in metropolitan Detroit, Michigan.
Antimicrob Agents Chemother. 2011 Feb; 55(2):593-9

McGrath EJ, Chopra T, Abdel-Haq N, Preney K, Koo W, Asmar BI, Kaye KS. 
An outbreat of carbapenems-resistant Acinetobacter baumannii infection in a neonatal intensive care unit:  investigation and control.
Infect Control Hosp Epidemiol.  2011 Jan;32(1): 34-41.

Hayakawa K, Marchaim D, Vidaillac C, Lephart P, Pogue JM, Sunkara B, Kotra H, Hasan A, Shango M, Yerramalla Y, Osunlana AM, Chopra T, Dhar S, Salimnia H, Tybak MJ, Kaye KS. 
Growing Prevalence of Vancomycin-Resistant Enterococcus Faecalis in the Region with the Highest Prevalence of Vancomycin-Resistant Staphylococcus Aureus.
Infection Control and Hospital Epidemiology, September 2011, Vol. 32 No. 9 pp. 922-924.

Marchaim D, Chopra T, Perez F, Hayakawa K, Lephart PR, Bheemreddy S, Blunden C, Hujer AM, Rudin S, Shango M, Campbell M, Varkey J, Slim J, Ahmad F, Patel D, Chen TY, Pogue JM, Salimnia H, Dhar S, Bonomo RA, Kaye KS. 
Outcomes and genetic relatedness of carbapenems-resistant Enterobacteriaceae at Detroit medical center.
Infect Control Hosp Epidemiol. 2011 Sep;32(9):861-71.

Chopra T, Chandrasekar PH, Salimnia H, Heilbrun LK, Smith D, Alangaden GJ. 
Recent epidemiology of Clostridium difficile infection during hematopoietic stem cell transplantation
Clin Transplant. 2011 Jan-Feb:25(1):E82-7.

Marchaim D , S lim J, Dhar S, Flanagan E, Chopra T, Farber M, Weaver D, Kaye KS.  
A regional survey of the use of mechanical bowel preparations prior to colorectal surgery.
Ann Surg. 2011 Apr;253(4):830-40.

Kaye KS, Marchaim D, Chen TY, Chopra T, Anderson DJ, Choi Y, Sloane R, Schmader KE. 
Predictors of nosocomial bloodstream infections in older adults.
J Am Geriatr Soc. 2011 Apr;59(4):622-7

Patel D, Husain M, Vidaillac C, Steed ME, Rybak MJ, Seo SM, Kaatz GW. 
Mechanisms of in-vitro-selected daptomycin-non-susceptibility in staphylococcus aureus.
Int J Antimicrob Agents. 2011 Nov;38(5):442-6. Epub 2011 Aug 12.

Sabatini S, Gosetto F, Manfroni G, Tabarrini O, Kaatz GW, Patel D, Cecchetti V.  
Evolution from a natural flavones nucleus to obtain 2-(4-Propoxypheny) quinoline derivatives as potent inhibitors of the S. aureus NorAefflux pump.
J Med Chem.  2011 Aug 25;54(16):5722-36.  Epub 2011 Aug 3.

Patel D, Crane LR. 
Growing Old with HIV.
Curr Infect Dis Rep. 2011 Feb;12(1):75-82.

Brincat JP, Carosati E, Sabatini S, Manfroni G , Fravolini A, Raygada JL, Patel D, Kaatz GW, Cruciani G. 
Discovery of Novel Inhibitors of the NorA Multidrug Transporter of Staphylococcus aureus.
J Med Chem. 2011 Jan 12;54(1): 354-65.  Epub 2010 Dec 8

Mitra S, Alangaden GJ. 
Recurrent Urinary Tract Infections in Kidney Transplant Recipients.
Curr Infect Dis Rep.  2011 Dec; 13(6):579- 87

Chandrasekar PH, Cavaliere R, Rust RS JR, Swaminathan S, Huff J, Talavera F, Lopate G, Roos K. 
Haemophilus Meningitis.
Medscape March 20, 2011


Reddy T, Chopra T, Marchaim D, Pogue JM, Alangaden, Salimnia H, Boikov D, Navon-Venezia S, Akins R, Selman P, Dhar S, Kaye KS.  
Trends in antimicrobial resistance of Acinetobacter baumannii isolates from a metropolitan Detroit health system.
Antimicrob Agents Chemother. 2010 May;54(5):2235-8.

Chen TY, Anderson DJ, Chopra T, Choi Y, Schmader KE, Kaye KS. 
Poor functional status is an independent predictor of surgical site infections due to Methicillin-resistant Staphylococcus aureus in older adults.
J Am Geriatr Soc. 2010 Mar;58(3):537-32.

Whby K, Chopra T, Chandrasekar P. 
Intravenous and inhalational colistin-induced respiratory failure.
Clin Infect Dis. 2010 Mar 15;50(6):e-38-40

Chopra T, Kaatz GW. 
Treatment strategies for infective endocarditis.
Expert Opin Pharmacother. 2010 Feb;11(3):345-60.

Chopra T, Bhargava A, Kumar S, Chopra A, Dhar S, Afonso L, Sobel JD
Candida kefyr endocarditis in a patient with hypertrophic obstructive cardiomyopathy.
Am J Med Sci. 2010 Feb;339(2):188-9

Kosmidis C, Levine DP. 
Daptomycin:  pharmacology and clinical use.
Expert Opin Pharmacother. 2010 Mar;11(4):615-25

Chopra T, Taormino M, Chandrasekar PH. 
Genital Ulcers Progressing to Fatal Sepsis During Treatment with All-Trans Retinoic Acid (ATRA) for Acute Promyelocytic Leukemia. 
Infectious Diseases in Clinical Practice. 2011 Jan; 19(1):70-71

Dhar S, Tansek R, Toftey EA, Dziekan BA, Chevalier TC, Bohlinger CG, Fitch M, Flanagen ME, Chopra T, Marchaim D, Kaye KS. 
Observer bias in hand hygiene compliance reporting.
Infect Control Hosp Epidemiol 2010 Aug;31(8):869-80.

Chopra T, Zhao JJ, Alangaden G, Wood MH, Kaye KS. 
Preventing surgical site infections after bariatric surgery: value of perioperative antibiotic regimens.
Expert Rev Pharmacoecon Outcomes Res. 2010 June; 10(3):317-28.

Sengstock DM, Thyagarajan R, Apalara J, Mira A, Chopra T, Kaye KS. 
Multidrug-resistant Acinetobacter baumannii: an emerging pathogen among older adults in community hospitals and nursing homes.
Clin Infect Dis 2010 Jun 15;50(12):1611-6.

Chopra T, Alangaden GJ,  Chandrasekar PH.  
Clostridium difficile infection in cancer patients and hematopoietic stem cell transplantation.
Expert Rev Anti Infect Ther. 2010 Oct;8(10):1113-9

Patel D, Kosmidis C, Seo SM, Kaatz GW. 
Ethidium bromide MIC screening for enhanced efflux pump gene expression or efflux activity in Staphylococcus aureus.
Antimicrob Agents Chemother. 2010 Dec;54(12):5070-3.

Swaminathan S, Alangaden GJ. 
Treatment of resistant enterococcal urinary tract infections.
Curr Infect Dis Rep. 2010, Nov,12(6):455-64


Raygada JL, Levine DP. 
Methicillin-Resistant Staphylococcus aureus:  A Growing Risk in the Hospital and in the Community.
American Health & Drug Benefits, 2009;2:86-95.

Frempong-Manso E, Raygada JL, DeMarco CE, Seo SM, Kaatz GW. 
Inability of a reserpine-based screen to identify strains overexpressing efflux pump genes in clinical isolates of Staphylococcus aureus.
Int J Antimicrob Agents, 2009 Apr;33:360-3.

Raygada JL and Levine DP. 
Managing CA-MRSA Infections:  Current and Emerging Options.
Infect Med, 2009; 26:49-58.

Chopra T, Chandrasekar PH. 
Pseudomonas dacryocystitis in a woman with ovarian cancer.
Infections in Medicine. Consultant Apr 2009

Sivasubramanian G, Siddiqui MF, Tangella K. 
Corticosteroid-Induced Scabies crustosa in an Elderly patient.
Hospital Physician.  Am J Med Sci 2012 Mar; 343(3):248

Sivasubramanian G and Zaman R. 
Osseous Blastomycosis of Foot Masquerading as Cellulitis.
Infectious Diseases in Clinical Practice July 2010 4:267-268

Sivasubramanian G and Sobel JD
Refractory Urinary Tract and Vulvovaginal Infection caused by Candida krusei.
Int Urogynecol J Pelvic Floor Dysfunct, 2009;20:1379-81.

Chopra T and Chandrasekar P. 
Salmon Colored Rash in a Young Woman.
Infectious Diseases in Clinical Practice. Infect Dis Clin Pract 2010;18:193-194

Ramesh MS, Chandrasekar PH. 
Challenges in the Current Medical Management of Invasive Fungal Infections in Hematopoietic Stem Cell Transplantation.
Expert Review of Anti-infective Therapy.  2009 Dec; 7(10):1151-3

Ramesh MS, Chandrasekar PH. 
Invasive Aspergillosis.
BMJ/ Point of Care.

Sivasubramanian G, Frempong-Manso E, MacArthur RD. 
Abacavir/ Iamivudine combination in the management of HIV:  a review. Therapeutics and Clinical Risk Management.
Ther Clin Risk Manag. 2010 Mar;36:83-94

Chen LF, Chopra T, Kaye KS. 
Chapter:  Pathogens Resistant to Antimicrobial Agents Epidemiology, Molecular Mechanisms, and Clinical Management.
Infect Dis Clin North Am. 2009 Dec;23(4):817-45

Sivasubramanian G and Sobel JD
Chapter:  Urinary Tract Infections Due to Aspergillus.  In: Aspergillosis:  From Diagnosis to Prevention
Pasquolotto A (Editor), Springer Science, Chapter 54, 2009.

Sivasubramanian G, Sobel JD
Refractory Urinary Tract and Vulvovaginal Infection Caused by Candida krusei.
Int Urogynecol J Pelvic Floor Dysfunct.  2009 Nov;20(11):1379-81

Chopra T, Gokhale RS. 
Polyketide versatility in the biosynthesis of complex mycobacterial cell wall lipids.
Methods Enzymol.  2009;459:259-94

Chen LF, Chopra T, Kaye KS. 
Pathogens resistant to antibacterial agents
Infect Dis Clin North AM. 2009 Dec:23(4):817-45


Ramesh M, Chandrasekar PH. 
Effective alternates to trimethoprim-sulfamethoxazole as antimicrobial prophylaxis in stem cell recipients:  Are there any? 
Pediatr Transplantation, 2008; 12:823-826.

Varghese GM, Sobel JD
Fungal Endocarditis.
Curr Infect Dis Rep, 2008;10:275-9

Huet AE Raygada JL, Mendiratta K, Seo SM and Kaatz GW. 
Multidrug Efflux Pump (MDR-EP) Overexpression (OE) in Staphylococcus aureus (SA) after Single and Multiple in vitro Exposures to Biocides and Dyes.
Microbiology, 2008;154:3144-3153.

Narreddy S, Chandrasekar PH. 
False-positive Aspergillus galactomannan (GM) assay in histoplasmosis.
J Infect, 2008;56:80-1.


Narreddy S, Mellon-Reppen S, Abidi MH, Klein JL, Peres E, Heilbrum LK, Smith D, Alangaden G, Chandrasekar PH. 
Non-bacterial infections in allogeneic non-myeloablative stem cell transplant recipients.
Transpl Infect Dis, 2007;9:3-10

Chen AY, Zervos MJ, Vazquez JA. 
Dalbavancin:  a novel antimicrobial.
Int J Clin Pract 2007; 61:853-63.

DeMarco CE, Cushing LA, Frempong-Manso E, Seo SM, Jaravaza TAA and Kaatz GW. 
Efflux-Related Resistance to Norfloxacin, Dyes, and Biocides in Bloodstream Isolates of Staphylococcus aureus
Antimicrob Agents Chemother, 2007;51:3235-3239.

Buhari MA, Varon N, Chandrasekar PH. 
CNS blastomycosis presenting as cerebral lesions in an HIV-seronegative homeless patient.
Infect Med, 2007;24:8(S)12-14


DeMarco CE, MacArthur RD. 
Battling Sepsis.
ADVANCE for Managers of Respiratory Care, 2006;15:30.

DeMarco CE, MacArthur RD. 
The importance of early and appropriate initial antimicrobial therapy.
Avances in Sepsis, 2006;5:60-1

Kaatz GW, DeMarco CE, Seo SM. 
MepR, a repressor of the Staphylococcus aureus MATE family multidrug efflux pump MepA, is a substrate-responsive regulatory protein.
Antimicrob Agents Chemother, 2006p;50:1276-81

Alangaden GJ, Thyagaranjan R, Gruber SA, Morawski K, Garnick J, el-Amm JM, West MS, Sillix DH, Chandrasekar PH, Haririan A. 
Infectiious complications after kidney transplantation:  Current epidemiology and associated risk factors.
Clin Transplant, 2006;20:401-409.

Krishnan S, Chandrasekar PH. 
Recurrent strongyloides stercoralis hyperinfection syndrome; Case report and Review of Literature.
Infectious Diseases in Clinical Practice, 2006;14:240-243


Louis JM, Buhari MA, Blackwell SC, Refuerzo J, Allen D, Gonik B, Jones TB. 
Characteristics associated with suboptimal viral suppression at delivery in human immunodeficiency virus-1-infected pregnant women.
Am J Obstet Gynecol 2005;193(3 Pt 2):1266-9

Krishnan S, Manavathu EK and Chandrasekar PH. 
A comparative study of the fungicidal activity of Voriconazole and amphotericin B against aspergillus fumigates hyphae.
J Antimicrob Agents and Chemother, 2005;55:914-920.